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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
Non Nmda Receptor Antagonist Cnqx, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
6 7 Dinitroquinoxaline 2 3 Dione, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
6 Cyano 7 Nitroquinoxaline 2 3 Dione Disodium Salt, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
Non Nmda Receptor Antagonist 6 Cyano 7 Nitroquinoxaline 2 3 Dione, supplied by Tocris, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 5. <t>CNQX,</t> but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.
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Figure 5. CNQX, but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.

Journal: The Journal of Neuroscience

Article Title: Granule Neuron Regulation of Purkinje Cell Development: Striking a Balance Between Neurotrophin and Glutamate Signaling

doi: 10.1523/jneurosci.18-10-03563.1998

Figure Lengend Snippet: Figure 5. CNQX, but not APV, rescues Purkinje cells from BDNF toxicity. A, Experiments with CNQX; B, experiments with APV. Purified Purkinje and granule cells were cocultured in serum-free medium for 6 or 14 div, with or without 10 ng/ml BDNF, 50 mM APV (an NMDA receptor antagonist), or 50 mM CNQX (a non-NMDA receptor antagonist). Pur- kinje cell survival is represented as a percentage of the Purkinje cell number in untreated control cultures at each time point. Error bars indicate SE. div, Days in vitro. Asterisks, Statistically significant differences from BDNF-treated cultures; **p, , 0.01.

Article Snippet: The NMDA receptor antagonist D-APV and the non-NMDA receptor antagonist CNQX were purchased from Tocris Cookson and used at 50 mM.

Techniques: Control, In Vitro